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Image Search Results
Journal: Molecular Metabolism
Article Title: Incretin-like effects of small molecule trace amine-associated receptor 1 agonists
doi: 10.1016/j.molmet.2015.09.015
Figure Lengend Snippet: TAAR1 is expressed in pancreatic β-cells and increases insulin secretion (A) Specific staining with anti-hTAAR1 Ab in human pancreas, duodenum and pylorus and (B) co-localization (yellow) of TAAR1 (green) with insulin (red) in human islets. Relative expression of TAAR1 in (C) human islets and (D) INS1E cells was detected by qRT-PCR, expression levels were normalized to GAPDH mRNA (+). In negative PCR controls (−) reverse transcription (RT) was omitted. (E) Increased insulin secretion in INS1E cells at elevated glucose (16 mM) concentration (white bars 2 mM, black bars 16 mM glucose) by RO5166017, a GPR40 agonist (GPR40) or Exendin-4 (Ex-4); n = 6. (F) Glucose-stimulated (11.2 mM glucose, black bars; 2 mM glucose, white bar) insulin secretion is increased by RO5166017 in human islets; n = 3. Dotted lines reflect glucose-induced insulin secretion without compound treatment. Bars in (E) and (F) represent mean ± standard deviation. Statistical analysis: One way Anova followed by Dunnett. ** p < 0.01, *** p < 0.001.
Article Snippet: Plasma insulin was determined by ELISA (Mercodia ® , Uppsala Sweden), total GLP-1 by
Techniques: Staining, Expressing, Quantitative RT-PCR, Reverse Transcription, Concentration Assay, Standard Deviation
Journal: American Journal of Physiology - Endocrinology and Metabolism
Article Title: Intestinal FFA3 mediates obesogenic effects in mice on a Western diet
doi: 10.1152/ajpendo.00016.2022
Figure Lengend Snippet: Expression levels of gut peptide hormones are altered by loss of intestinal FFA3. Ileal gene expression levels of incretin hormones Gcg, Pyy, Cck , and Gip ( A). Colonic gene expression levels of incretin hormones Gcg, Pyy, Cck , and Gip ( B ). Plasma levels of GLP-1 ( C ) and GIP ( D ) measured 15 min after an oral bolus of glucose. For A and B, n = 4–7 per group. For C and D , n = 6–10 per group. Values represent means ± SE, * P < 0.05 vs. respective genotypes on control diet (CD), and “#” indicates a significant difference between Western diet groups at a # P > 0.05 calculated using one-way ANOVA.
Article Snippet: Plasma GLP-1 levels and gastric inhibitor protein (GIP) levels were quantified by
Techniques: Expressing, Gene Expression, Clinical Proteomics, Control, Western Blot